Pluripotent stem cells and livestock genetic engineering

Induced Pluripotent Stem Cells: Challenges and Opportunities

Regenerative capacity of mammals is limited and can rarely regenerate a specific organ or tissue fully. Due to these limitations, regenerative medicine seeks efficient and safe cell sources for regeneration of damaged tissues and organs or treatment for incurable diseases. Human embryonic stem cells (HESCs) hold two important properties called self renewal and pluripotency. However, the use of ...

Genome engineering in human pluripotent stem cells

Human pluripotent stem cells (hPSCs) hold great promise for modeling and recapitulation of human biological processes. Coupled with the advent of genome engineering tools, specifically the CRISPR–Cas9 systems, hPSCs have opened a multitude of possibilities in modeling human biology and creating novel cellular therapies. Here, we review the fundamentals of both technologies, and the wide-ranging...

Advances in homology directed genetic engineering of human pluripotent and adult stem cells.

The ability to introduce precise genomic modifications in human cells has profound implications for both basic and applied research in stem cells, ranging from identification of genes regulating stem cell self-renewal and multilineage differentiation to therapeutic gene correction and creation of in vitro models of human diseases. However, the overall efficiency of this process is challenged by...

induced pluripotent stem cells: challenges and opportunities

regenerative capacity of mammals is limited and can rarely regenerate a specific organ or tissue fully. due to these limitations, regenerative medicine seeks efficient and safe cell sources for regeneration of damaged tissues and organs or treatment for incurable diseases. human embryonic stem cells (hescs) hold two important properties called self renewal and pluripotency. however, the use of ...

Genetic Engineering of Livestock Improvement